michael davidson Search Results


memerald paxillin  (Addgene inc)
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Addgene inc memerald paxillin
Memerald Paxillin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfp n1 plasmid  (Addgene inc)
95
Addgene inc egfp n1 plasmid
Egfp N1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid encoding mkeima red mito 7  (Addgene inc)
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Addgene inc plasmid encoding mkeima red mito 7
Plasmid Encoding Mkeima Red Mito 7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sfgfp n1  (Addgene inc)
93
Addgene inc sfgfp n1
Sfgfp N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mtagbfp2 tomm20 n 10 plasmid  (Addgene inc)
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Addgene inc mtagbfp2 tomm20 n 10 plasmid
Mtagbfp2 Tomm20 N 10 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcardinal h1 10  (Addgene inc)
88
Addgene inc mcardinal h1 10
Mcardinal H1 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transfection emerald dectin1a n 10  (Addgene inc)
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Transfection Emerald Dectin1a N 10, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mneptune2 c1 vector  (Addgene inc)
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Addgene inc mneptune2 c1 vector
Mneptune2 C1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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meos2  (Addgene inc)
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Addgene inc meos2
Meos2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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memerald arp2 c 14  (OriGene)
92
OriGene memerald arp2 c 14
( A ) Top panel-representative image of a cell expressing GFP-Rab35 and tagRFP-DENNd1c. Bottom panel-representative image of a cell expressing GFP-Rab35 and <t>Emerald-Arp2.</t> ( B ) Western blot of DENNd1a-c knockdown. For each blot a DENNd1 was knocked down and the remaining two DENND1s were probed for to test for compensation effects. ( C ) Representative live-image of a cell expressing GFP-Rab11 and TagRFP647 (647)-LifeAct before and after CK-666 treatment. ( D ) GFP-Rab35 and LifeAct-647 co-expression in sprout live-imaged at baseline and following treatment with CK-666. Arrowheads indicate accumulations of GFP-Rab35 and LifeAct-647. Dotted line indicates sprout exterior. ( E ) Quantification of GFP-Rab35 localization upon DMSO (vehicle) or CK-666 administration. Apical plasma membrane (PM, uniformly localized to apical membrane), apical PM accumulation (Rab35 puncta at the apical membrane), cytosolic (localized in the cytoplasm), cytosolic accumulations (Rab35 puncta in the cytoplasm), equal PM (Rab35 equally distributed between the apical and basal membranes). Two-dimensional localization of GFP-Rab35 with globular-actin and filamentous-actin. ( F ) Representative image of a cell expressing GFP-Rab35 and stained for filamentous (F) and globular (G) actin. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.
Memerald Arp2 C 14, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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memerald arp2 c 14 - by Bioz Stars, 2026-05
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michael davidson  (Addgene inc)
93
Addgene inc michael davidson
( A ) Top panel-representative image of a cell expressing GFP-Rab35 and tagRFP-DENNd1c. Bottom panel-representative image of a cell expressing GFP-Rab35 and <t>Emerald-Arp2.</t> ( B ) Western blot of DENNd1a-c knockdown. For each blot a DENNd1 was knocked down and the remaining two DENND1s were probed for to test for compensation effects. ( C ) Representative live-image of a cell expressing GFP-Rab11 and TagRFP647 (647)-LifeAct before and after CK-666 treatment. ( D ) GFP-Rab35 and LifeAct-647 co-expression in sprout live-imaged at baseline and following treatment with CK-666. Arrowheads indicate accumulations of GFP-Rab35 and LifeAct-647. Dotted line indicates sprout exterior. ( E ) Quantification of GFP-Rab35 localization upon DMSO (vehicle) or CK-666 administration. Apical plasma membrane (PM, uniformly localized to apical membrane), apical PM accumulation (Rab35 puncta at the apical membrane), cytosolic (localized in the cytoplasm), cytosolic accumulations (Rab35 puncta in the cytoplasm), equal PM (Rab35 equally distributed between the apical and basal membranes). Two-dimensional localization of GFP-Rab35 with globular-actin and filamentous-actin. ( F ) Representative image of a cell expressing GFP-Rab35 and stained for filamentous (F) and globular (G) actin. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.
Michael Davidson, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Top panel-representative image of a cell expressing GFP-Rab35 and tagRFP-DENNd1c. Bottom panel-representative image of a cell expressing GFP-Rab35 and Emerald-Arp2. ( B ) Western blot of DENNd1a-c knockdown. For each blot a DENNd1 was knocked down and the remaining two DENND1s were probed for to test for compensation effects. ( C ) Representative live-image of a cell expressing GFP-Rab11 and TagRFP647 (647)-LifeAct before and after CK-666 treatment. ( D ) GFP-Rab35 and LifeAct-647 co-expression in sprout live-imaged at baseline and following treatment with CK-666. Arrowheads indicate accumulations of GFP-Rab35 and LifeAct-647. Dotted line indicates sprout exterior. ( E ) Quantification of GFP-Rab35 localization upon DMSO (vehicle) or CK-666 administration. Apical plasma membrane (PM, uniformly localized to apical membrane), apical PM accumulation (Rab35 puncta at the apical membrane), cytosolic (localized in the cytoplasm), cytosolic accumulations (Rab35 puncta in the cytoplasm), equal PM (Rab35 equally distributed between the apical and basal membranes). Two-dimensional localization of GFP-Rab35 with globular-actin and filamentous-actin. ( F ) Representative image of a cell expressing GFP-Rab35 and stained for filamentous (F) and globular (G) actin. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.

Journal: bioRxiv

Article Title: Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting Angiogenesis

doi: 10.1101/2022.01.13.476231

Figure Lengend Snippet: ( A ) Top panel-representative image of a cell expressing GFP-Rab35 and tagRFP-DENNd1c. Bottom panel-representative image of a cell expressing GFP-Rab35 and Emerald-Arp2. ( B ) Western blot of DENNd1a-c knockdown. For each blot a DENNd1 was knocked down and the remaining two DENND1s were probed for to test for compensation effects. ( C ) Representative live-image of a cell expressing GFP-Rab11 and TagRFP647 (647)-LifeAct before and after CK-666 treatment. ( D ) GFP-Rab35 and LifeAct-647 co-expression in sprout live-imaged at baseline and following treatment with CK-666. Arrowheads indicate accumulations of GFP-Rab35 and LifeAct-647. Dotted line indicates sprout exterior. ( E ) Quantification of GFP-Rab35 localization upon DMSO (vehicle) or CK-666 administration. Apical plasma membrane (PM, uniformly localized to apical membrane), apical PM accumulation (Rab35 puncta at the apical membrane), cytosolic (localized in the cytoplasm), cytosolic accumulations (Rab35 puncta in the cytoplasm), equal PM (Rab35 equally distributed between the apical and basal membranes). Two-dimensional localization of GFP-Rab35 with globular-actin and filamentous-actin. ( F ) Representative image of a cell expressing GFP-Rab35 and stained for filamentous (F) and globular (G) actin. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.

Article Snippet: The following constructs were procured for this study: GFP-Rab35 S22N inactive (gift from Peter McPherson; Addgene plasmid # 47426); GFP-Rab35 WT (gift from Peter McPherson, Addgene plasmid # 47424); GFP_Rab35 Q67L (gift from Peter McPherson, Addgene plasmid # 47425); mEmerald-Fascin-C-10 (gift from Michael Davidson, Addgene plasmid # 54094); pARF6(Q67L)-CFP (gift from Joel Swanson, Addgene plasmid # 11387); pARF6(T27N)-CFP (gift from Joel Swanson, Addgene plasmid # 11386); pARF6-CFP (gift from Joel Swanson, Addgene plasmid # 11382); pcDNA3-HA-human OCRL (gift from Pietro De Camilli, Addgene plasmid # 22207); pCDNA3.0_mitoLAMA-G97 (gift from Kai Johnsson, Addgene plasmid # 130705); pGST1-GGA3-VHS (gift from James Hurley, Addgene plasmid # 44420); mEmerald-ARP2-C-14 (gift from Michael Davidson, Addgene plasmid # 53992); MICAL-L1 (Origene, RG214051); and DENNd1c (Origene, RC206410);

Techniques: Expressing, Western Blot, Staining

( A ) Two-dimensional localization of GFP-Arp2 with DENNd1c (top panels) and GFP-Rab35 (bottom panels). ( B ) Representative images of DMSO and CK-666 (Arp Inhibitor) treated sprouts expressing GFP-Rab35. L denotes lumen. ( C,D ) Live imaging of GFP-Rab35 or tagRFP-DENNd1c with TagRFP647-LifeAct at baseline and after treatment with CK-666. White arrowheads denote disappearance of Rab35 puncta over time. ( E ) Representative live-images of a cell expressing mCherry-Arp2 and GFP-Rab35 before and after CK-666 treatment White arrowheads denote disappearance of Rab35 puncta over time. ( F ) Cartoon of a mitochondria-localized GFP-nanobody and controlled release of GFP-Rab35 upon treatment with Trimethoprim (TMP). In the absence of TMP the nanobody sequesters GFP or GFP-tagged proteins. In the presence of TMP the GFP cargo is released. ( G ) Live-image of a cell expressing GFP-Rab35, TagRFP647 (647)-LifeAct and ligand-modulated antibody fragments targeted to the mitochondria (mito-LAMA) before and after TMP administration. ( H ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA before and after TMP administration. ( I ) Live-image of a cell expressing GFP-Rab35, TagRFP-DENNd1c and mito-LAMA before and after TMP administration. ( J ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA before and after TMP administration and then treated with CK-666. ( K ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA treated with DENNd1c siRNA (si) before and after TMP administration. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.

Journal: bioRxiv

Article Title: Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting Angiogenesis

doi: 10.1101/2022.01.13.476231

Figure Lengend Snippet: ( A ) Two-dimensional localization of GFP-Arp2 with DENNd1c (top panels) and GFP-Rab35 (bottom panels). ( B ) Representative images of DMSO and CK-666 (Arp Inhibitor) treated sprouts expressing GFP-Rab35. L denotes lumen. ( C,D ) Live imaging of GFP-Rab35 or tagRFP-DENNd1c with TagRFP647-LifeAct at baseline and after treatment with CK-666. White arrowheads denote disappearance of Rab35 puncta over time. ( E ) Representative live-images of a cell expressing mCherry-Arp2 and GFP-Rab35 before and after CK-666 treatment White arrowheads denote disappearance of Rab35 puncta over time. ( F ) Cartoon of a mitochondria-localized GFP-nanobody and controlled release of GFP-Rab35 upon treatment with Trimethoprim (TMP). In the absence of TMP the nanobody sequesters GFP or GFP-tagged proteins. In the presence of TMP the GFP cargo is released. ( G ) Live-image of a cell expressing GFP-Rab35, TagRFP647 (647)-LifeAct and ligand-modulated antibody fragments targeted to the mitochondria (mito-LAMA) before and after TMP administration. ( H ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA before and after TMP administration. ( I ) Live-image of a cell expressing GFP-Rab35, TagRFP-DENNd1c and mito-LAMA before and after TMP administration. ( J ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA before and after TMP administration and then treated with CK-666. ( K ) Live-image of a cell expressing GFP-Rab35, mCherry-Arp2 and mito-LAMA treated with DENNd1c siRNA (si) before and after TMP administration. Insets are areas of higher magnification. All experiments were done using Human umbilical vein endothelial cells in triplicate.

Article Snippet: The following constructs were procured for this study: GFP-Rab35 S22N inactive (gift from Peter McPherson; Addgene plasmid # 47426); GFP-Rab35 WT (gift from Peter McPherson, Addgene plasmid # 47424); GFP_Rab35 Q67L (gift from Peter McPherson, Addgene plasmid # 47425); mEmerald-Fascin-C-10 (gift from Michael Davidson, Addgene plasmid # 54094); pARF6(Q67L)-CFP (gift from Joel Swanson, Addgene plasmid # 11387); pARF6(T27N)-CFP (gift from Joel Swanson, Addgene plasmid # 11386); pARF6-CFP (gift from Joel Swanson, Addgene plasmid # 11382); pcDNA3-HA-human OCRL (gift from Pietro De Camilli, Addgene plasmid # 22207); pCDNA3.0_mitoLAMA-G97 (gift from Kai Johnsson, Addgene plasmid # 130705); pGST1-GGA3-VHS (gift from James Hurley, Addgene plasmid # 44420); mEmerald-ARP2-C-14 (gift from Michael Davidson, Addgene plasmid # 53992); MICAL-L1 (Origene, RG214051); and DENNd1c (Origene, RC206410);

Techniques: Expressing, Imaging